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Evaluating Endothelial Cell Health

By Louise DeRiso posted 02-15-2014 18:38

  

The Endothelium in Diabetes

 

It is well known that cardiovascular disease (CVD) is a major cause of morbidity and mortality for individuals with diabetes.  The common conditions that coexist with type 2 diabetes, such as hypertension and dyslipidemia, are clear risk factors for CVD.  There have been a number of studies that have shown the efficacy of controlling individual cardiovascular risk factors in preventing or slowing CVD.

Researchers are studying the role of endothelial dysfunction as a possible mechanism by which Type 2 diabetes increases the risk of CVD.

The endothelium, a single layer of cells that lines the heart and blood vessels, is responsible for maintaining vascular homeostasis.  Endothelial cells cover a surface area larger than the skin, and form a barrier between a blood vessel’s lumen and its smooth muscle lining.  The endothelium is metabolically active, and releases nitric oxide and other vasoactive substances that influence thrombosis, fibrinolysis, antiproliferation, and the inflammatory response.

Researchers have been interested in the vascular endothelium and its role in the development of heart disease and diabetic retinopathy.  Endothelial dysfunction, a condition in which the blood vessels are unable to dilate fully when needed, is thought to be one the earliest signs of atherosclerosis.  Research indicates that endothelial dysfunction tends to develop before the blood vessel walls start to thicken and before atherosclerotic plaques begin to appear on them.  The dysfunction of the endothelium may cause atherosclerosis by promoting stiffening of the blood vessels, adhesion of platelets to the inner walls of blood vessels, inflammation, and blood clotting. 

The health of the endothelium can be assesses either invasively, or by noninvasive methods.  One invasive method is a quantitative coronary angiography, which involves injecting acetylcholine into the vasculature.  If the endothelium is healthy, the vessels relax.  Endothelial dysfunction is indicated by vessel constriction.  Various noninvasive tests include Doppler echocardiogram, positron emission tomography and magnetic resonance imaging.  The flow-mediated dilation of the brachial artery is a simple test that is conducted at the Vascular Clinical and Translational Center (VCTRC) at the University of Pittsburgh.  The procedure includes placement of a blood pressure cuff on a patient’s arm, inflating the cuff above the systolic pressure for five minutes, and releasing the cuff, and using Doppler flow to look at the reactive hyperemia that occurs.  If blood vessels dilate as the blood rushes back, this indicates that the endothelium is healthy.  If the endothelium is dysfunctional, the blood vessels do not respond. 

In addition to promotion of a healthy lifestyle, by the reduction of modifiable risk factors, research is being conducted on improving the health of the endothelium, and ultimately, reducing the risk of heart disease.  One area of study is the development of drugs (anti-VEGFs) that block the receptors within the endothelium that tend to promote the growth of new blood vessels. The receptors on the endothelial cells tend to promote angiogenesis, with the help of growth factors such as vascular endothelial growth factor (VEGF).  These cells can adversely affect diabetic retinopathy by promoting the development of abnormal new blood vessels in the retina. Diabetic retinopathy may be stopped or delayed with the use of drugs that block the action of VEGF. 

A healthy endothelium is necessary to maintain vascular homeostasis and to reduce the risk of cardiovascular disease.  It is likely that the endothelium’s influence on the cardiovascular system will become more important in clinical medicine. With increased understanding of the endothelium’s crucial role in disease and health, the diabetes educator can help people with diabetes adopt healthy lifestyles.

 

Louise DeRiso, MSN, CRNP, CDE

Nurse Manager, Vascular CTRC

University of Pittsburgh

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02-21-2014 07:37

Evaluating Endothelial Cell Health

Very enlightening atricle!!  Does the same premis hold true for people without diabetes?  Our 47 year old son just had a heart attack and I wonder if this info also reflects his situation.

02-19-2014 11:19

Your Blog

Louise


Thank you for a such a well written description of the pathophysiology.


Pat Adams

02-16-2014 14:41

Evaluating Endothelial Cell Health

Louise,


 


I can only imagine how interesting and inspiring your work must be! Diabetes Care stated insulin resistance is strongly associated with decreased nitrous oxide and endothelial dysfunction. We certainly do what we can to promote a healthy lifestyle in the face of what seems to me ever increasing insulin resistance and obesity.


 


January 7, 2014, Diabetes Insight was reporting a breakthrough in treating macular edema in an interview with David M. Brown, MD, Clinical Asso­ciate Professor, Department of Oph­thalmology, Methodist Hospital-Weill Cornell Medical College.””The RISE and RIDE trials, which led to the approval of ranibizumab (anti-VEGF) for diabetic macu­lar edema, showed that the average patient gained ≈1.5 lines (ie, 8 letters) of vision within 1 week of receiving the injection. At the end of the trial, ≈40% of patients gained 3 lines, which was a major improvement over focal laser therapy.” Improvements in delivery are being studied as patients need retreatment every month or two as the medication wears off.


Lastly, my favorite and perhaps most immediately applicable in the treatment of endothelial dysfunction is a study from Amsterdam, the Netherlands, from the European Society of Cardiology September 1, 2013 in 74 patients with stable CAD. Professor Deljanin Ilic said: “In the setting of cardiovascular risk factors and cardiovascular disease the endothelium loses its normal function.1 Since endothelium derived nitric oxide is necessary to maintain an adequate vascular response, correction of endothelial dysfunction has become a goal of therapy.” She continues the article with this: “The combination of music and exercise training led to the most improvement in endothelial function. Improvements in endothelial function were associated with significant improvements in exercise capacity.”



She added: “Listening to joyful music for 30 minutes has been associated with improved endothelial function, possibly by β-endorphin mediated activation of endothelium derived nitric oxide.2 The vascular health benefits of music may be due to endorphins or endorphin like compounds released from the brain when we hear music we like.”

Professor Deljanin Ilic concluded: “Listening to favorite music alone and in addition to regular exercise training improves endothelial function and therefore may be an adjunct method in the rehabilitation of patients with CAD. There is no an ‘ideal’ music for everybody and patients should choose music which increases positive emotions and makes them happy or relaxed.”


 


I hope we are doing Zumba this year at conference! Just look at the benefits.


(Italics are mine)


Thank you for bringing this important and most significant topic of research to light!


 


Lisa Laird